| 1 |  Birmingham Legion FC | |
| 2 |  Brooklyn | |
| 3 |  Charleston Battery | |
| 4 |  Colorado Springs Switchbacks FC | |
| 5 |  Detroit City FC | |
| 6 |  El Paso Locomotive FC | |
| 7 |  FC Tulsa | |
| 8 |  Hartford Athletic | |
| 9 |  Indy Eleven | |
| 10 |  Las Vegas Lights FC | |
| 11 |  Lexington SC | |
| 12 |  Loudoun United FC | |
| 13 |  Louisville City FC | |
| 14 |  Miami FC | |
| 15 |  Monterey Bay F.C. | |
| 16 |  New Mexico United | |
| 17 |  Oakland Roots SC | |
| 18 |  Orange County SC | |
| 19 |  Phoenix Rising FC | |
| 20 |  Pittsburgh Riverhounds SC | |
