| 1 |  Appalachian | |
| 2 |  Asheville City SC | |
| 3 |  Austin FC | |
| 4 |  AV Alta | |
| 5 |  Ballard | |
| 6 |  Birmingham Legion FC | |
| 7 |  Carolina Core | |
| 8 |  CD Faialense | |
| 9 |  Charleston Battery | |
| 10 |  Charlotte FC | |
| 11 |  Charlotte Independence | |
| 12 |  Chattanooga FC | |
| 13 |  Chattanooga Red Wolves SC | |
| 14 |  Chicago Fire FC | |
| 15 |  Colorado Springs Switchbacks FC | |
| 16 |  Columbus Crew 2 | |
| 17 |  Corpus Christi | |
| 18 |  DC United | |
| 19 |  Des Moines Menace | |
| 20 |  Detroit City FC | |
